STUDY OF POST-VACCINAL IMMUNITY IN LABORATORY ANIMALS IMMUNIZED WITH LIVE PLAGUE VACCINE FROM STRAIN EV
DOI:
https://doi.org/10.52269/22266070_2024_4_9Keywords:
EV strain , immunity, plague, vaccine , white miceAbstract
The article presents the results of experiments conducted to study humoral immunity by indirect hemagglutination reaction (IHR) and cellular immunity using the method of local hemolysis to detect antibody-forming cells (ABC) according to Yerne and Nordin and the method of rosette formation of T-lymphocytes in laboratory animals immunized with dry live plague vaccine EV. The IHR test results revealed that by the 90th day post-vaccination, antibody titers in animals from Group 2, vaccinated with a dose equivalent to 20 man/doses for mice, and in animals from Group 3, vaccinated with a dose equivalent to 25 man/doses for mice, were identical.
The studies conducted using the local hemolysis method to detect ABCs showed that the parenteral administration of a camel pox vaccine to white mice, at a dose equivalent to 18 man/doses, induced the production of ABCs at a level four times higher, and at 20 man/doses, five times higher than with a dose of 15 man/doses. Increasing the vaccine dose to 25 man/doses did not result in a statistically significant increase in the number of ABCs produced.
The study of antigen-specific rosette formation in dynamics revealed an early increase in this index, with the highest level (12.2) recorded on the 21st day of the study in animals vaccinated with a dose equivalent to 20 man/doses.
