DEVELOPMENT OF A TEST SYSTEM FOR RAPID DIAGNOSIS OF TRICHINELLOSIS IN ANIMALS

Abstract

Trichinosis is a parasitic disease caused by nematodes of the Trichinella genus, posing significant concerns for both veterinary and medical fields. This disease is widespread in many countries, including Kazakhstan. Trichinosis primarily spreads through domestic and wild animals, and humans can contract it through raw or undercooked meat. In recent years, the spread of trichinosis in Asia, Europe, and other regions has shown an increasing trend. In Kazakhstan, the disease is mainly transmitted through dog, badger, and wild boar meat. Traditional diagnostic methods, such as trichinoscopy and serological techniques (ELISA, immunoblotting), are used for diagnosing the disease, but these methods require sophisticated equipment and considerable time. Therefore, the research objective was to develop an immunochromatographic test system for the rapid detection of trichinosis. During the study, trichinella antigens and antibodies were obtained, and their activity was investigated. Colloidal gold-labeled conjugates were prepared, and their effectiveness was tested using enzyme immunoassay (ELISA). The results demonstrated that the Protein G conjugate exhibited high activity with trichinella antibodies. The effectiveness of the developed immunochromatographic test system was tested on samples from experimentally infected animals and wild animals naturally infected with the disease. The test system showed high sensitivity and efficiency, providing reliable results compared to commercial test systems. In conclusion, the research results confirmed the effectiveness of the immunochromatographic test system for the rapid diagnosis of trichinosis. This method allows for the quick and accurate detection of trichinosis in rural and remote areas.