PRODUCTION OF ANTIGEN FOR DIAGNOSIS OF BOVINE CAMPYLOBACTERIOSIS USING AGGLUTINATION REACTION

Abstract

The article presents the results of experiments conducted to get a sensitive antigen for the diagnosis of сampylobacteriosis. The conducted studies established the correspondence of the Campylobacter fetus fetus B-0115 KazNIVI strain to the subspecies Campylobacter fetus subsp. fetus (Cff), it has been proven that when typing with diagnostic monospecific agglutinating sera of types I, II, III, it has a positive reaction with type II serum and a negative reaction with type I and III sera.

A method has been developed for getting somatic antigen from this strain, based on boiling the bacterial mass obtained by the original method for 60 min at 100⁰C. When performing a test tube agglutination reaction using the antigen we obtained, agglutination of somatic O-antigens is observed in lower titers compared to whole-cell OH-antigens, which indicates the specificity of somatic antigens for each subspecies of campylobacteria and confirms that the cultures belong to one or another type.

Based on the studies, Campylobacter fetus fetus B-0115 KazNIVI strain, characteristic of the fetus subspecies, was isolated, and a technology for producing a sensitive somatic antigen from it was developed. It was scientifically proven that the use of this antigen enables the diagnosis of campylobacteriosis in cattle using a test-tube agglutination reaction.