ANALYSIS OF BACTERIAL DIVERSITY OF SOILS TREATED WITHMINIMAL AND ZERO SOIL TECHNOLOGIES

Abstract

Two tillage systems were studied in crop rotation:

1. Minimal technology. The use of a Glyphosate non-selective herbicide herbicide in a fallow in the first half of summer and mechanical tillage in the second half. Application of combined tools on grain fields within crop rotation, excluding autumn treatments.
2. Zero soil technology. Mechanical tillage is completely excluded. Only direct seeding is performed using stubble drills equipped with hoe boots. Weed control in fallow fields is performed using exclusively the Glyphosate non-selective herbicide. In all crop rotation fields, spring soft wheat of the Omskaya 18 variety was sown, with a seeding rate of 3.5 million viable seeds per hectare.

Objective: To perform a comparative analysis of the diversity of 16S rRNA gene fragment sequences, amplified from metagenomic DNA, to determine the composition and structure of the soil bacteriobiome, comparing minimal and zero soil tillage technologies.

Task: For each sample, analyze the dependency between the number of identifiable OTUs and the total number of sequences to assess the feasibility of accurately comparing the bacterial biodiversity of the samples.

The evaluation of soil bacteriobiome composition and structure under different tillage methods revealed the main components of the bacteriobiome and their contributions.