STUDY OF BIOLOGICAL PROPERTIES OF B. ANTHRACIS STERNE 34 F2 STRAIN ON VARIOUS NUTRIENT MEDIA

Abstract

Frequent inoculation of reference strains and subcultures during strain storage leads to a change in the 
morpho-physiological properties of the pathogen, so it is important to study the main biological properties of 
live reference vaccine strains during long-term storage. As is known from literary sources, R- and S-forms 
differ in their morphological characteristics, the R-form produces long threads, the S-form produces single or 
paired rods. Type R in broth gives a scaly deposit, but the broth remains transparent, whereas type S results 
in uniform turbidity of the broth with an amorphous deposit. On agar, R-form colonies look like jellyfish heads, 
and S-form colonies are flat with smooth edges. The study on the main biological properties of B. anthracis 
variants is of practical interest for the production of veterinary drugs. It has been established that under 
favorable environmental conditions, spores pass into a vegetative form and revert to spores again in early 
autumn. In the body of animals and humans, anthrax bacilli form capsules. Capsules also form in culture media 
containing blood, serum, egg white or brain tissue. 
As a result of the studies, the biological properties of the B. anthracis Sterne 34 F2 strain were 
determined in various nutrient media, with an assessment of the morpho-physiological characteristics. 
The study used microbiological research methods, including inoculation and control of cultivation in 
nutrient media. 
The practical significance of the study is stemmed from optimization of the production of vaccines and 
diagnostic test systems, allowing for maximum production of antigen components.