ACETYCHOLINESTERASE AND ITS ROLE IN ERYTHROGRAM FORMATION IN METALLOTOXICOSIS

Abstract

The informative value of acetylcholinesterase activity in assessing changes in the shape, size and number of red blood cells under conditions of low-dose intake of lead and cadmium compounds into the animal body was studied. It was found that with toxicosis, the number of red blood cells decreased (in group I by 47.01%, in group II by 52.71%, in group III by 29.12%), determining the direction of changes in hemoglobin and hematocrit. The degree of shifts when using a metal cocktail was less pronounced, which reflects the presence of antagonistic relationships between metals. The value of the calculated parameters of the erythrogram (MSN, MSNS, MCV) indicated an increase in the volume of red cells (macrocytosis), an increase in the amount of hemoglobin in them while maintaining or slightly reducing the "packaging" of protein in cells. The activity of acetylcholinesterase in erythrocyte hemolysate decreased during the experiment and most significantly with the introduction of a metal cocktail (by 56.37%). An enzyme with lead toxicosis statistically significantly correlated with hemoglobin (r=0.73-0.92 ft), with cadmium - with the number of erythrocytes (r=0,82-0,83) and hemoglobin (r=0,88), with lead and cadmium - with the number of erythrocytes (r=0.79-0,86), hemoglobin (r=0,76-0,79) and MCH (r= - 0,66 − -0,74).