ON THE RESULTS OF THE STUDY OF SNP POLYMORPHISMS IN HORSES OF THE LOCAL BREED ZHABE OF THE KAZAKHSTAN POPULATION

Abstract

The authors of the article carried out genotyping of horses of the local breed to the toad KH «Kyzylsok» of the Zhambyl district of the Almaty region in the amount of 46 heads for the following gene loci: LCORL, PRKAG3 and B3GALNT2. It was found that the loci of the LCORL, PRKAG3 genes in the studied animals turned out to be polymorphic, i.e. genetic variants CC (89.13%), CG (10.87%) were identified, similar results were obtained for the second locus of the PRKAG3 gene, animals with the homozygous CC genotype prevail in this group (95.23%), the frequency of the heterozygous CT genotype was 4 .77%. For both studied loci, no other homozygous GG and TT genotypes were detected in the studied horses, there is also a violation of gene balance, alleles have a high frequency - С=-0.94, С=0.97, extremely low alleles: G=0.06 and T=0.03. The level of homozygosity in experimental animals was determined, for the LCORL locus - this indicator was 89.13%, for the second gene PRKAG3 - 95.23%, the level of heterozygosity was 10.87% and 4.77%, respectively. No heterozygous carriers of the deleterious mutation B3GALNT2 were found in horses of the local breed by PCR-RFLP analysis. We recommend that, in the SNP perspective, the BIEC2-808543 polymorphisms in the LCORL gene and AAWR_02017454:g.121684T>C in the coding part of the PRKAG3 gene be used as a DNA marker of meat and reproductive function in horses.