QUANTITATION AND EXTRACTION OF INULIN FROM THE ROOTS OF KOK-SAGHYZ (TARAXACUM KOK-SAGHYZ RODIN)

Abstract

This article provides the results of the extraction and quantitative determination of inulin from the roots of the kok-saghyz dandelion (Taraxacum kok-saghyz Rodin). Along with natural rubber, dandelion kok-saghyz is a producer of inulin. Inulin, in turn, is a sugar substitute, flavor enhancer for foods intended for diabetics, and is used in medicine. Seeds obtained from three populations of kok-saghyz dandelions; Saryzhaz, Kegen and Tuzkol were grown in Almaty. In this work, experiments were carried out to determine and obtain inulin from the dry roots of kok-saghyz at the end of the growing season. The main task was to obtain the greatest amount of inulin from the roots. At the end of September, the content of inulin reached its maximum value, 24%. This was 282.0 kg/ha, based on the expected yield of inulin based on extraction from 500,000 plants per hectare. Whereas the content of rubber is 22% by dry weight of the root. Two main methods for obtaining inulin from the roots were used in the work: enzymatic hydrolysis with HPLC analysis of the obtained monosaccharides, determination of inulin by the difference between fructosides and fructosans with quantitative determination of the total content of polyfructans in terms of fructose.